Journal: IBRO Neuroscience Reports

Article Title: Trimethylamine-N-oxide damages astrocytes and lymphatic endothelial cells in the cerebral lymphatic system

doi: 10.1016/j.ibneur.2025.09.001

Figure Lengend Snippet: Effects of TMAO on intracranial inflammation and functional proteins in animals. Levels of IL-6, NLRP3, TNF-α, and SNCA in the cerebrospinal fluid detected by ELISA. (B) The injury of ventral midbrain neurons detected by Nissl staining. (C) Immunofluorescence was used to detect the effects of TMAO on AQP4, GFAP, S100, claudin-5, and Ocln in the ventral midbrain of mice. Three animals were randomly selected from each group (n = 10) for sample testing, analysis of variance followed by LSD or Dunnett's post-hoc test was used to compare between-group differences. 'ns' indicates that there was no significant difference between the groups.

Article Snippet: After TMAO administration, mice were anesthetized with ketamine at a dose of 100 mg/kg by intraperitoneal injection, then stereotactically injected with 2.5 μL (concentration 3 μg/mL) recombinant human SNCA protein (PRS-pro-393, Amyjet Scientific Technology Co., Ltd, Hubei, China) intracranially at a rate of 0.25 μL/min.

Techniques: Functional Assay, Enzyme-linked Immunosorbent Assay, Staining, Immunofluorescence

Journal: bioRxiv

Article Title: Intracellular delivery of full-length antibodies via organ targeted lipid nanoparticles

doi: 10.1101/2025.09.26.678781

Figure Lengend Snippet: (a) Schematic depicting intracranial (i.c.) administration of Cy5-SynO4-SL4-LNPs into the substantia nigra (SN). (b) Representative images of coronal brain tissue sections from the SN region imaged 72 hours after i.c. injection. Sections were fixed and immunostained for tyrosine hydroxylase (TH, dopamine neurons, red), human phosphorylated alpha synuclein (pAS, green), and counterstained with DAPI for cell nuclei (blue). Scale bar = 5 mm. (c) Representative images of brain tissue sections fixed and immunostained for tyrosine hydroxylase (TH, dopamine neurons, red), human phosphorylated alpha synuclein (pAS, green), and counterstained with DAPI for cell nuclei (blue). Colocalization images indicates target engagement of Cy5-SynO4 (purple) with pAS (green). Scale bar = 20 µm. (d) Schematic depicting procedure for formulating transferrin-targeted LNPs (TF-Cy5-SynO4-SL4 LNPs) via microfluidic mixing. (e) Schematic depicting intravenous (i.v.) administration of TF-Cy5-SynO4-SL4 LNPs. (f) Representative images of control brain (non-injected) sections and treated brain sections 6 hours after i.v. injections. Tissue sections were fixed and immunostained for TH (dopamine neurons, red), pAS (green), and counterstained with DAPI for cell nuclei (blue). Cy5-SynO4 antibodies are indicated where they localized and co-localized with pAS in the SN. Scale bar = 50 µm. Results obtained from n = 3 independent repetitions ( n = 9 replicates each).

Article Snippet: An in-house ELISA assay using the direct ELISA method was used for the Anti-Human SNCA Therapeutic (SynO4) Antibody (TAB-0750CLV-L; Creative Biolabs, USA).

Techniques: Injection, Drug discovery, Control